Mouse Embryonic Fibroblasts (MEFs) are criticalÂ biological reagents in stem cell research. Used as “feeder cells”Â to maintain Embryonic Stem (ES) cells and induced Pluripotent Stem (iPS), theÂ quality of MEFs strongly influences Stem cell culture conditions, and may react differently depending on culture conditions.Â In parallel, MEFs can also be reprogrammedÂ into a variety of more matureÂ differentiated functionalÂ cell types (cardiomyocytes, neurons…). DuringÂ daily discussions withÂ Stem cell biologists, I have been able to collect Frequently Asked Questions and technical tips related to MEFs.
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