A previous post explained how samples obtained from patients treated with mAbs can show interference with WB & ELISA methods. More rarely, we can also find other interference effects caused by different factors.
Immunoassay interference is typically caused by non-specific interactions between antibodies within the assay itself and endogenous antibodies present within a patient’s sample. Any type of interference can cause an assay to report a false result. This can be avoided by using a blocking reagent, which is a product designed to prevent interfering antibodies from adversely affecting immunoassay results.