Self-renewal and differentiation capacities into multiple cell-types are 2 main characteristics of Stem Cells. Contrarily to Embryonic Stem Cells, Adult Stem Cells (ASC) are already committed to a differentiated lineage. Nevertheless, ASC still retain capacities of generating various cell types within their restricted lineage. It’s thus crucial to be able to access high quality and reliable research tools when tracking cell differentiation. Here, we’ll review the most popular reagents used to characterize the ectodermal lineage.