In this third post of the miRNA-related series, let’s take a look at miRNA overexpression.
Short RNAs are very prone to enzymatic degradation. Novel chemical technologies now allow RNA stabilization to make them resistant to cellular RNAse attacks. However, this approach remains optimal for short term assays. LNA (locked nucleic acid) oligonucleotides show extended half life in cells, characterized by their ability to bind complementary nucleic acids with exceptional affinity. To be mentioned also, 2′-O-Me nucleotide-composed oligonucleotides – as they show increased stability against nucleases and RNA dimers with 2′-O-Me oligos are not recognized by RNAse H (which is a big advantage for antisense technologies).