CRISPR-Cas or TALEN genome editing – which one to choose?
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR-associated) and Transcription Activator-Like Effector Nuclease (TALEN) are endonuclease based technologies aimed at developing targeted genome editing technologies.
CRISPR and TALEN provide Scientists with unique discovery tools for pathophysiology or genotype-phenotype studies by creating cellular models with gene knock-out, knock-in or tagging, promoter swapping, nucleotide substitution, protein truncation, reading frame disruption, modification of regulation by miRNA, genetic defect corrections…But, which one is the best for your application?
CRISPR or TALEN?
Which criteria to take into consideration to select TALEN over CRISPR or CRISPR over TALEN?
In a tech note, Ed Davis (Genome Editing expert at GenoCopoeia) compares TALEN and CRISPR tehnologies.
Ed also defines multiple criteria to consider when selecting the most appropriate gene editing method suited to the application of interest (specificity, efficiency, ease of use, methylation sensitivity,off-target effects…).
Download now the Technical Bulletin “Genome editing: Which should I choose, TALEN or CRISPR” written by Ed Davis!
See TALEN and CRISP-Cas9 genome editing technologies in action!