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Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
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Drug Discovery, Supplying Discovery Tools

How to measure Calcium in cell based assays?

30/06/2014 by Ali El Baya, PhD No Comments

In Pharma screening departments there is a strong need for Ca2+ measurement methods.

Why is this?

GPCR - Calcium mobilisation

GPCRs (G Protein Coupled Receptors) are still major targets of pharmaceutical screenings.

Around 30-40% of all drugs on the market are directed against GPCRs.

Drugs have an impact on the GPCR activity and thus the downstream signal transduction cascade. GPCRs are linked to the activity of heterotrimeric G proteins which consist of alpha, beta and gamma subunits. These G proteins have an effect on PhosphoLipase C (PLC) which leads to the release of Inositol Phosphate 3 (IP3) which finally leads to intracellular Ca2+ mobilization (release from the endoplasmic reticulum ) – Ca2+ finally has effect on a number of further cellular processes.

The fact that GPCR activation always leads to Ca2+mobilization is commonly used to indirectly measure the impact of compounds/drug candicates on GPCRs.

Hence the need for Ca2+ measurement methods, such as the following…

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Specific cytokines & growth factors in… hematology!

by Elodie Monin No Comments
Growth Factors & cytokines

Hematology is the study of blood, blood-forming organs and blood diseases. The formation of blood cells is referred to as hematopoiesis. All cellular components of the blood are derived from hematopoietic stem cells.

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