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  • Home
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    • ADME-Tox
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Cell Sourcing - Cell Culture Technologies, Supplying Discovery Tools

Struggling with tissue dissociation?

13/06/2014 by Jean-François Têtu, PhD No Comments
Technical tips icon by tebu-bio

Human liver Myofibroblasts VimentinSuccessful culture of primary cells requires obtaining a maximum yield of functionally viable single cells from whole tissues. The primary factors that affect successful dissociation of whole tissue have to do with tissue origin, species, age of the animal, the dissociation medium and enzyme(s) used, the amount of impurities in any crude enzyme preparation, the concentration(s) of enzyme(s), and the temperature and incubation times. While factors dealing with type of tissue and species cannot be controlled, conditions associated with tissue dissociation are manageable.

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Cell Sourcing - Cell Culture Technologies, Drug Discovery, Headlines, Supplying Discovery Tools

Isogenic Knockout iPSC Lines for Modeling of CNS Disorders

by Jean-François Têtu, PhD No Comments
Live staining astrocytes derived from knock‐in reporter line for GFAP

Live staining astrocytes derived from knock‐in reporter line for GFAP

Somatic cell reprogramming technology enables researchers to build disease models by generating iPSC lines from patient samples, to differentiate them into targeted primary cells, and manifest disease symptoms in vitro.

So far, many disease models for CNS disorders, such as Alzheimer’s disease (AD), Parkinson’s disease (PD), Autism, Schizophrenia, and Amyotrophic Lateral Sclerosis (ALS), have been generated using this strategy. However, there is a common limitation for disease models solely based on patient‐derived iPSC lines: the mechanism of action of genetic mutations underlying a disease is often complicated by diverse genetic backgrounds from patient to patient since most genetic mutations associated with CNS disorders have in general small effect on the disease phenotype.

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Modulate hepatic inflammation

by Jean-François Têtu, PhD No Comments

Kupffer cells are macrophages endogenous to the liver which have the ability to modulate hepatic inflammation and injury associated with various pathophysiologies and toxicities. Pro-inflammatory cytokines released by activated Kupffer cells, such as TNF-α and IL-6, are associated with up-regulation of acute-phase response proteins and suppression of CYP enzymes.

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Cell Sourcing - Cell Culture Technologies

Primary cell sourcing: Cryopreserved Human Adult Sebocytes

by Philippe Fixe, PhD No Comments
tebu-bio primary cell sourcing Human Primary Sebocytes Zenbio

Sebocytes are emerging cellular models to study skin biology but also to design new compounds to regulate acne, seborrhoea and other sebaceous gland-related diseases. Easy access to cryopreserved and well-qualified sources of Human Adult Sebocytes will boost skin research and compound testing projects.

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