tebu-bio & Cellix are pleased to invite you to their 2nd workshop dedicated to the VenaFlux platform. This workshop will be structured around both theoretical presentations and practical sessions.

The presentations will not only cover introduction of our biochips, pumping solutions and analysis software, which enable to accurately mimic shear stress/shear flow rates found in vivo,  but also presentations by users giving concrete data on cell rolling, adhesion and migration to antibody-coated or endothelial cell-cultured microcapillaries under venous or arterial physiological flow.

The lab sessions will be organised at tebu-bio Laboratories and run by Cellix’s experts. We will start with system configuration and assay set-up, following which the attendees will perform two different experiments (see program). After debriefing, the results will be analysed and we will interpretate the data.

To know more: https://sites.google.com/a/tebu-bio.com/2nd-flow-assay-workshop/

AKT (Protein Kinase B or PKB), is a serine/threonine-specific protein kinase with known key involvement in glucose metabolism, apoptosis, cell proliferation, transcription and cell migration.

In this study, the authors investigated the modulatory effects of LIM-only protein FHL2/DRA/SLIM3 in phenotypic changes of Smooth Muscles Cells (SMC) in SMC-rich lesion formation.
They have found that FHL2 regulates SMC’s functions during vascular proliferative diseases by modulating Cyclin D1 expression via ERK 1/2 signaling pathway.

Congratulations to the authors, who turned to tebu-bio’s range of products when selecting their growth factors to culture aortic SMC for in vitro scratch-wound and migration assays.

Want to know more?

Source: Karakula K. et al. “The LIM-Only Protein FHL2 Reduces Vascular Lesion Formation Involving Inhibition of Proliferation and Migration of Smooth Muscle Cells” (2014) 9(4): e94931. doi:10.1371/journal.pone.0094931

Related research products:

• A complete offer of active Cytokines and Growth factors  and Chemokines PeproTEch including recombinant Mouse PDGF-BB.
• A characterized source of primary Smooth Muscle Cells (SMC) from Human, Mouse, Rat, Bovine origin.
• FHL2 research reagents (from primary antibodies to siRNA, expression vectors and, Gene Reporter Assays, miRNA…).
• Transwell-free in vitro ORIS migration assays.

Oris Cell Migration Assay Video