Study of living tissues has gained wide spread application in microscopy with the advent of fluorescent dyes and inverted microscopes dedicated to their viewing. A critical component is maintaining physiological conditions in media formulations, including pH and osmolarity, without contributing to background fluorescence. Most cells are grown in media formulations that use bicarbonate buffers, requiring the continuous presence of CO2 to maintain physiological pH. Just 15 minutes outside a CO2 incubator can change the pH from 7.4 to 8.1, and to 8.7 after an hour.

Cell reprogramming of Mouse and Human somatic cells to induced Pluripotent Stem Cells (iPSC) have opened outstanding new opportunities for biomedical research. iPSC are now becoming unique cellular models for personalized therapies and regenerative medicine.

Researchers from the FANTOM Consortium and the RIKEN PMI and CLST mapped transcription start sites and their usage in human and mouse primary cells, cell lines and tissues to produce a comprehensive overview of mammalian gene expression across the human body.

http://www.nature.com/nature/journal/v507/n7493/full/nature13182.html

Analysis of Autofluorescence in Polymorphonuclear Neutrophils: A New Tool for Early Infection Diagnosis by Monsel A et al.

Source: Monsel A et al. (2014) Analysis of Autofluorescence in Polymorphonuclear Neutrophils: A New Tool for Early Infection Diagnosis. PLoS ONE 9(3): e92564. doi:10.1371/journal.pone.0092564

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