• Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
  • Facebook
  • LinkedIn
  • Twitter
  • Vimeo
Home
Research areas
    ADME-Tox
    Biomarkers
    Cell Biology and Signalling
    Cell Sourcing - Cell Culture Technologies
    Drug Discovery
    Gene Expression - Molecular Biology
    Stem Cells
    Supplying Discovery Tools
Contact us
Meet the authors
Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Cell Sourcing - Cell Culture Technologies, Supplying Discovery Tools

Lower background fluorescence in live cell imaging

28/03/2014 by Jean-François Têtu, PhD No Comments

Study of living tissues has gained wide spread application in microscopy with the advent of fluorescent dyes and inverted microscopes dedicated to their viewing. A critical component is maintaining physiological conditions in media formulations, including pH and osmolarity, without contributing to background fluorescence. Most cells are grown in media formulations that use bicarbonate buffers, requiring the continuous presence of CO2 to maintain physiological pH. Just 15 minutes outside a CO2 incubator can change the pH from 7.4 to 8.1, and to 8.7 after an hour.

Continue reading
Cell Biology and Signalling, Supplying Discovery Tools

iPS cells: Bioactive small molecules make exogenous reprogamming genes dispensable!

by Philippe Fixe, PhD No Comments
Bioactive small molecules from tebu-bio

Cell reprogramming of Mouse and Human somatic cells to induced Pluripotent Stem Cells (iPSC) have opened outstanding new opportunities for biomedical research. iPSC are now becoming unique cellular models for personalized therapies and regenerative medicine.

Continue reading
News

A promoter-level mammalian expression atlas

by Jean-François Têtu, PhD No Comments

Researchers from the FANTOM Consortium and the RIKEN PMI and CLST mapped transcription start sites and their usage in human and mouse primary cells, cell lines and tissues to produce a comprehensive overview of mammalian gene expression across the human body.

http://www.nature.com/nature/journal/v507/n7493/full/nature13182.html

 

News

Autofluorescence in Polymorphonuclear Neutrophils: A New Tool for Early Infection Diagnosis (Monsel A et al. – PLoS One 2014)

by Philippe Fixe, PhD No Comments
Lympholyte
Analysis of Autofluorescence in Polymorphonuclear Neutrophils: A New Tool for Early Infection Diagnosis by Monsel A et al.
In this study, the authors demonstrates that the average autofluorescence changes of the PolyMorphonuclear Neutrophils (PMNs) during bacterial infections, can serve as a surrogate marker of infection. The autofluorescence is related to NAD(P)H pathway in PMNs.

Source: Monsel A et al. (2014) Analysis of Autofluorescence in Polymorphonuclear Neutrophils: A New Tool for Early Infection Diagnosis. PLoS ONE 9(3): e92564. doi:10.1371/journal.pone.0092564

Congratulations to the authors who have relied on tebu-bio when selecting their discovery tools.

Want to know more about Lympholyte to isolate human Polymorphonuclear Neutrophils?

Lympholyte-poly is a ready-made sterile and endotoxin tested solution which is a mixture of sodium metrizoate and Dextran 500 that allows for the isolation of human polymorphonuclear granulocytes from whole blood.

Most popular posts

  • HeLa cells: Origin of this important cell line in life science research
  • From RUO to IVD - the acronym guide to reagents' intended use
  • How to choose the perfect buffer to get a pure, stabilised, functional protein
My Tweets

Privacy & Cookies: This site uses cookies. By continuing to use this website, you agree to their use.
To find out more, including how to control cookies, see here: Cookie Policy

Copyright © 2018 - tebu-bio - visit our main website at tebu-bio.com