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Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
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Cell Sourcing - Cell Culture Technologies, Supplying Discovery Tools

295 solutions for isolating primary cells

20/03/2014 by Jean-François Têtu, PhD No Comments
Primary cells, active enzymes and small molecules for stem cell and Drug discovery research

Human Pancreatic Islets - Prodo LaboratoriesIt is widely accepted that data obtained from primary cells is not only desired, but, most relevant when trying to study physiological interactions. Experimentation in primary cells allows a deeper and more physiologically relevant view into the cellular function of proteins and enzymes that will allow the design of more specific drugs with meaningful and specific targets.

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Cell Sourcing - Cell Culture Technologies, Supplying Discovery Tools

Stem cell-derivative Motor Neurons / Muscle fiber co-culture functional bio-assay

by Philippe Fixe, PhD No Comments
Human Induced Pluripotent (HIP™) Neural cells

Chipman et al. (Dalhousie University, Halifax, Nova Scotia, Canada) have developed a new cell-based system “mimicking” in vitro Mature Neuromuscular Junction (NMJs). (1)

Chipman et al. PLOSOne (2014) DOI: 10.1371/journal.pone.0091643

Chipman et al. PLOSOne (2014) DOI: 10.1371/journal.pone.0091643

NMJs play an important role in the functionality of Motor neurons.

Their dysfunction is seen in the early stages of various Motor Neuron Diseases (MNDs) like the Amyotrophic Lateral Sclerosis (ALS), Spinal Muscular Atrophy (SMA), Primary Lateral Sclerosis (PLS), Progressive Muscular Atrophy (PMA)…

To meet these needs, Chipman et al. have created a clever co-culture system using Embryonic Stem Cell-derived Motor Neurons (ESCMNs) with primary myotubes. They also reported that NCAM-/- ESCMN/myofiber co-cultures exhibit the same phenotypes observed in NCAM-/- mice.

This work give hopes to study MNDs in vitro by using motor neurons differentiated from induced Pluripotent Stem cells (iPS) derived from individuals with ALS and SMA.

Want to know more about cell-based assays and co-culture systems?

(1) Chipman et al. “A Stem-Cell Based Bioassay to Critically Assess the Pathology of Dysfunctional Neuromuscular Junctions” (2014) PLOSone, March 13. DOI: 10.1371/journal.pone.0091643

tebu-bio provide European researchers with high quality and innovative tools for:

  • Stem cell studies & reprogramming.
  • Ion channel analysis with Smartox venomous synthetic peptides (ex. Omega-AGATOXIN IVA a blocker of P/Q-type calcium channel (Cav2.1)).
  • Drug discovery with pure and affordable active small molecules from FOCUS Biomolecules (ex. Nifedipine a L-type Ca channel blocker).
  • Primary and iPS cells

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