Low oxygen tension, or Hypoxia, regulates numerous cellular and tissular functions. In cancer research, hypoxia is a key regulator of tumor development, aggressiveness and therapy resistance by acting on malignant cells and their microenvironment. Hypoxia is also involved in age-related diseases and acts through intracellular and intercellular cascade of events (exosomes, paracrine loops, angiogenesis…) (1-2).
They regulate gene expression by interfering with the chromatin structure and the binding of effector proteins to post-translational modified histones (1).
Several experimental strategies to analyze such binding have recently been designed; histone modified peptide arrays being one of them.