Self-renewal and differentiation capacities into multiple cell-types are 2 main characteristics of Stem Cells. Contrarily to Embryonic Stem Cells, Adult Stem Cells (ASC) are already committed to a differentiated lineage. Nevertheless, ASC still retain capacities of generating various cell types within their restricted lineage. It’s thus crucial to be able to access high quality and reliable research tools when tracking cell differentiation. Here, we’ll review the most popular reagents used to characterize the ectodermal lineage.

Ectoderm and cell-type lineage with appropriate differentiation biomarkers - GeneTex and tebu-bio

Ectodermal biomarkers. Source: GeneTex and tebu-bio.

Found in various tissues of the adult body, ASC are composed of various lineage-engaged cell types. Issued from the ectoderm, Neural Stem Cell (NSC) and Epidermal Stem Cell (ESC) types are among the most characterized ones.

Many antibody-based or fluorescent-based research tools are now available to conveniently measure the differentiation of Stem cells and iPS cells (iPSC). A few examples:

Let’s take a look at the most popular research antibodies used by researchers seeking to further characterize NSC and ESC in immuno-assays. This selection will lead you to lists of high quality antibodies with user-friendly filters to help you find the antibodies suited to your applications (WB, IP, ICC, IF, FCM, ELISA…).

Neural Stem Cells (NSC) biomarkers

FoxA2 has been recently identified as a pivotal transcription factor in the specification and final differentiation of the DA phenotype and the differentiation of human ESC into DA neurons. Therefore, antibodies related to the Fox transcription factor family are more and more popular in stem cell research.

In the adult, NSCs play an important role in the repair of the Central Nervous System and the neurological functions. One might prefer to characterize NSC by identifying intracellular or surface biomarkers.

Here’s our selection:

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NCS surface biomarkers

NSC intracellular biomarkers

 Epidermal Stem Cells (ESC) biomarkers

ESC brings optimal skin homeostasis, wound-repair, and regenerative capacities. To follow the development of ESC, biomarkers analysed can be the following:

What about you?

As ASC knowledge evolves, many other new biomarkers are identified. Which markers are you tracking for your studies concerning Neural and Epidermal Stem Cells? Looking for a specific biomarker in a particular immuno-assay? Leave your comment here!

Written by Philippe Fixe, PhD
Philippe Fixe is a former Marketing Manager at tebu-bio, passionate about innovation and R&D in Life sciences, Biotechnology, Medical research, Drug discovery, and also a keen photographer.