Cell-Based ELISA Kits allow now a precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.
I have communicated on previous posts regarding our high density glass slide antibody arrays services. I will focus here on another antibody microarray product line, the C-series from Raybiotech, based on a membrane antibody array technology. Similar to western blotting hence very easy to process, they are suitable to most research laboratories for targeted protein profiling.
Signal-Seeker assays (Cytoskeleton Inc.) provides researchers with the most comprehensive reagents enabling highly sensitive and specific detection of the key Post Translational Modifications (PTMs) of your Protein of Interest. In this post, I invite you to look at experimental results obtained with these unique assays for the characterization of Tyrosine Phosphorylation, Ubiquitination, SUMOylation and Acetylation of endogenous proteins.
In a few forthcoming posts, I’d like to share several short articles published by Dr Chris Bohl of Sekisui Xenotech, a technical expert in the field of ADME-Tox tools and applications. I feel they may be of special interest to researchers working in the field of ADME-Tox studies. Today, we’ll take a look at compounds […]
Sekisui Xenotech’s Research BioBank provides you with normal and diseased human liver tissue samples for scientific investigation. Tissues are collected with the initial intent for transplant, thus making them perfect candidates to advance basic medical science and contribute to the development of new treatments. The care taken to preserve viability of the tissue intended for […]
Sekisui XenoTech now offers human liver tissue microarrays (TMA) and a microsomal pool for studying and developing new treatments for fatty liver disease (FLD). The microsomes and arrays feature non-alcoholic steatohepatitis (NASH) and other liver tissue from the company’s Research Biobank . The initiative is part of Sekisui XenoTech’s ongoing commitment to furthering knowledge of hepatic […]
Over the last years, mRNA-based experimental approaches have been successfully used in a broad range of research applications (genome editing, gene replacement, vaccine, immunotherapy, cellular reprogramming…) without risk of integration into the genome of the host cell. mRNA molecules, mimicking fully processed, capped and polyadenylated mRNA, can now be reliably produced via in vitro transcription […]
In living organisms, ExtraCellular Matrix (ECM) builds up a complex and dynamic network of molecules, playing a major role in structural support, adhesion, movement and cellular regulation. Major components of the ECM (Collagen, Proteoglycans, Elastin, Hyaluronan) are key elements studied in the Pharmaceutical and Cosmetology fields due to their strong involvement in tissue regeneration, skin […]
Cell-based assays have become a classic way to monitor cells’ reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is Firefly Luciferase, combined or not with Renilla Luciferase. Luciferases allow simple data acquisition on a plate reader. Nevertheless, Firefly Luciferase requires cell lysis. End-point […]
Hyaluronic acid (also called HA or Hyaluronan) is a glycosaminoglycan with elevated viscosity, enabling tissues (eye, skin, joint and synovial fluid…) to resist to physical and mechanical constraints (torsion, flexion…). Over time, when HA production declines, tissues progressively lose these tensile properties, leading to wrinkles and fold, weak re-epithelisation and age-related troubles. But HA is also involved in many other […]
Over the years, Sekisui XenoTech has built up a strong expertise in preparing subcellular fractions for ADME applications. There is a list of the fractions readily available for prompt delivery across Europe. However, the researcher’s specific needs in terms of species model, characterization or tissue for a particular metabolism study may not be met by the […]