As is already known, multiple families of small GTPases (Ras, Arf and Rho families) make up the Ras GTPase superfamily. Due to their role as molecular switches in cellular signal transduction (active GTP-bound state vs inactive GDP-bound states) and in many other cellular responses (cytoskeletal reorganization, regulation of transcription, apoptosis…), these small GTPases are the subject […]
Latest Articles
Neurons, Astrocytes, Microglia: High Quality primary cells source
In the field of neurosciences, increasing research is being undertaken on neurodegenerative disorders like Parkinson’s and Alzheimer’s diseases, as well as on nerve injury and regeneration. One of the challenges researchers face is finding the appropriate cellular neuromodels, as close as possible to the in vivo environment. Primary cells are one of the answers, increasingly […]
Cancer immunotherapy: explore the cytokine “milieu” with Quantibody 200 Biomarker Service
Autologous tumour cell vaccines vs. dendritic cell vaccines: What’s best, and why? Looking at the cytokine “milieu” with the Quantibody 200 Biomarker Microarray Service helped the authors in understanding the differences in vaccine responses.
HDACs: Active drug targets for Immunotherapy treatment
For many pathologies such as cancer or neurological disorders, it has been found that Histone Deacetylase (HDAC) activity is disrupted, making them promising targets for drug development. Scientists need access to a broade range of products ( Active Enzyme, inhibitors/activators, Biochemical screening assays…), to simplify their research and identification of HDAC inhibitors.
Antibody reproducibility: what will make the difference…
Rockland Immunochemicals, Inc., (Rockland) provide the highest quality antibodies to the academic, biopharma, and diagnostic industries for use in basic research, assay development, preclinical and clinical studies, and bioprocessing. With facilities in Pennsylvania established since 55 years ago, Rockland manufactures products ideally suited for integration into critical assays such as western blotting, immunohistochemistry (IHC), immunofluorescence […]
Maximize metabolic activities by limiting Hepatocyte cryoinjury
I am following up on my series of posts based on the work done by Chris Bohl and collaborators at Sekisui Xenotech. Here, he looks at the effect of cryopreservation on pooled hepatocytes metabolic activities, and how the patented Cryostax technology can address this issue.
Live Cell Imaging – Watch Human Osteosarcoma cells in action, stained with SiR-Tubulin probes
As you may already know, Spirochrome have developed a broad range of Live Cell Imaging probes for the staining of DNA, Lysosome, F-Actin and Microtubules.
DDI studies: New TransiPort cells for OATP1A2 and OATP2B1 transporters
Membrane transporters can have clinically relevant effects on the pharmacokinetics and pharmacodynamics of a drug in various organs and tissues by controlling its absorption, distribution and elimination. Together with metabolizing enzymes, they can drive a drug’s pharmacological action, as well as a drug can modulate transporter expression or activity, hence the importance of evaluating transporter-mediated drug-drug […]
NADPH RapidStart regeneration system for long-term metabolism
NADPH is a critical cofactor supporting numerous biochemical reactions. In ADME-Tox studies, NAD(P)H regeneration is strongly recommended when using drug metabolizing enzymes (ex. Cytochrome P450 (CYP), Flavin-containing MonoOxygenases (FMO)), Recombinant CYPs (incl. bactosomes) or cellular fractions (Microsomes, S9). Currently, the most simple and cost-effective way to regenerate the NAD(P)H in situ and enzymatically is to use the commercially-available RapidStart™ NADPH Regenerating System (Xenotech-Sekisui). RapidStart uses an […]
New: QuicGSH3.0 – a fluorescent probe to quantify intracellular glutathione
Glutathione (GSH) is a tri-peptide (Glutamic acid, Cystein and Glycine) present in cells, showing an antioxidant effect allowing the reduction and the removal of Reactive Oxygen Species (ROS) and Peroxide from the cell. The new QuicGSHS3.0 probe from Goryo Chemicals will allow you to easily quantify intracellular concentration of GSH by Fluorescent Live cell Imaging.
10 Human Immune Checkpoint biomarkers quantified simultaneously
Immune checkpoint molecules play an important role in T cell functionality after TCR/MHC signalling. Blockade of two B7/CD28 family checkpoint molecules, CTLA-4 and PD-1, have already demonstrated excellent efficacy in increasing T cell responses to a variety of tumours. Identification of novel target and new checkpoint blockade remains a key element in Drug Discovery (See […]