Human Peripheral Blood Mononuclear Cells (hPBMCs) are essential for designing cellular models to be used in cell therapy and drug discovery research programs [1-7, 12]. Being able to access reliable and ethical sources of well-characterized hPBMCs is now becoming fundamental for running physiologically relevant cell-based assays. A good opportunity to discuss the expanding applications for […]
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cAMP signals visualized in live human pancreatic primary cells
In a recent publication, researchers from the University of Miami Miller School of Medicine (USA) describe that Serotonin released by human beta cell inhibits glucagon secretion by alpha cells. They demonstrated that this paracrine loop was mediated via the cAMP pathway. To do so, they captured in live human pancreatic islet cells cAMP signals using […]
Microcell arrays – obtain more accurate data from single living cells
Understanding how mammalian cells function requires a dynamic perspective. Recent improvements in our abilities to perform fluorescence microscopy on primary cells, coupled with advances in pipelines for quantifying and extracting data, have made possible a better understanding of the temporal complexity of cell signalling pathway. Due to the heterogeneity seen in both eukaryotic and prokaryotic cell […]
Analysis tool for direct and sandwich ELISA – convenient and reliable
Many ELISA kits – one analysis tool Analyte quantification (in pg/ml or ng/ml) such as IL-6, TNFalpha and numerous other proteins is conveniently performed with immunoassays called ELISA kits. At tebu-bio, we not only provide more than 10 000 ELISA kits but we also support researchers through our techserv team who are on-hand to suggest […]
Cell Signalling: Ras-Raf-MEK-ERK pathway research tools
The Ras-Raf-MEK-ERK Pathway is an important cell signalling route with many implications for cancer biology and therapeutic development. Dis-regulation of some of this pathway’s proteins expression and phosphorylation status are observed in about one-third of all human cancers. Access to specific tools to study this pathway is essential to a better understanding of its role […]
Optimizing the CAS9 messenger RNA for vector-free gene editing
Sequence engineering and chemical modification of CAS9 mRNA For transgenesis and therapeutic perspectives, vector-free delivery of the CAS9 for gene editing is highly preferable. It avoids the risk of hitting the genome due to the vector integration. Directly using CAS9 mRNA is a top option. Furthermore, as a new promising class of therapeutic biologics, messenger […]
Stable Cas9-expressing cell lines
Shen et al (Nature Methods, 2017) explore and identify synthetic interactions among 73 cancer-associated genes. To perform their loss of function screen they combined CAS9-expressing cell lines with a sgRNA library of high titer lentiviral particles. Most of these gene interactions were subsequently validated by drug treatment.
New range of Pig P450s Bactosomes
If you are looking for enzymatic in vitro tools for drug metabolism, reaction phenotyping, metabolite generation, using pig as model, you may find these newly released Pig Bactosomes from Cypex of interest. Bactosomes are recombinant enzymes expressed in E. coli with a patented expression system that enables expression of drug metabolising enzymes without the need […]
New in Live Cell Imaging: CopperGREEN and GlycoGREEN probes
One of our aims is to offer you, the researcher, a large and varied selection of biologically relevant, innovative Live Cell Imaging tools. In 2016, we started a collaboration with Goryo Chemical, when our specialists selected their unique fluorescent dyes for cellular analysis, assays and live cell imaging. In one of my previous posts, I […]
Metabolite production made fast and easy – BMO Kits
Metabolite characterization can be a lengthy process making your in vitro drug testing time consuming and expensive. Whether you are working on phase I or phase II metabolic enzymatic reactions, take a look at these BMO kits which can help you speed up this characterisation process. The Phase I BMO metabolism kits take you through […]
Single-domain antibodies: Novel Llama immunoreagents
A single-domain antibody (sdAb, also called Nanobody) is an antibody fragment consisting of a single monomeric variable antibody domain. Like a whole antibody, it is able to bind selectively to a specific antigen. With a molecular weight of only 12–15 kDA, single-domain antibodies are much smaller than common antibodies (150–160 kDa). They have been shown […]