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5 Preps EUR 24
24€
50 Preps EUR 129
129€
200 Preps EUR 477
477€


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E.Z.N.A.® Endo-Free Plasmid Mini Kit II

Product name
E.Z.N.A.® Endo-Free Plasmid Mini Kit II
Catalog number
D6950-00 (5 Preps)
D6950-01 (50 Preps)
D6950-02 (200 Preps)
Description
Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid Mini Kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<1 EU/ug) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane. 
Source
Omega Bio-tek
Product category
Genomics
Product sub category
DNA Extractions
Biological activity
 
Shipment info
Room Temperature
Nacres Codification
NA.55

Protocol

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