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1ml EUR 5310
5310€


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ATA-Phe-Phe-Arg-CMK

Product name
ATA-Phe-Phe-Arg-CMK
Catalog number
IINFFRCK-1ml (1ml)
Description
ATA-FFRCK (Na-[(acetylthio) acetyl]-D-Phe-Phe-Arg-CH2Cl) and ATA-FPRCK (Na-[(acetylthio)acetyl]-D-Phe-Pro-Arg-CHCl2) are active-site specific labeling reagents for serine proteases. They are derivatized from irreversible peptide chloromethyl ketones and facilitate incorporation of a thioester moiety via alkylation of the catalytic site Histidine residue. Liberation of the free thiol group is accomplished by gentle treatment with hydroxylamine (NH2OH) following irreversible incorporation into the enzyme catalytic site. The free thiol then becomes a site for specific modifications with thiol-reactive probes such as iodoacetamide fluorescent probes. Many serine proteases in which free thiols are lacking may be specifically labeled at the active site by these reagents. Both ATA-FPRCK and ATA-FFRCK have been used to label thrombin with 5-(iodoacetamido) fluorescein (5-IAF). The probe was then effectively utilized to follow conformational changes in the catalytic domain of alpha-thrombin upo 
Source
Innovative Research
Product category
Cells, Media & Fractions
Product sub category
Plasma/Serum/Complement
Shipment info
Dry Ice
Nacres Codification
NA.61

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