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30mL EUR 3633
3633€


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3CLglow Assay - 30mL

Product name
3CLglow Assay - 30mL
Catalog number
C1160G-30mL (30mL)
Description
The 3CLglow Assay is a 3CLpro (3C-like protease, Mpro, or Main protease) inhibitor assay, is a new fluorescent Live Cell assay providing a robust, biologically meaningful tool to evaluate therapeutic drugs that target SARS-CoV-2 replication.

The 3CLglow Assay kit includes the 3CLpro enzyme and a fluorescent biosensor for monitoring 3CLpro enzyme activity. This live cell assay bridges the gap between in vitro biochemical assays and living cells and organisms. To create a robust assay for 3CLpro activity in living cells, we added an inhibitor tag to the bright mNeonGreen fluorescent protein. In the absence of 3CLpro, the protein is not fluorescent. A 3CLpro cleavage site is included that separates the inhibitor tag from the fluorescent protein. The result is a biosensor that lights up when 3CLpro activity is present in the cell. 
Source
Montana Molecular
Product category
Expression Vectors
Product sub category
ORF expression
Shipment info
Cool Pack
Nacres Codification
NA.51

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Protocol

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