PUROTOXIN 1
- Product name
- PUROTOXIN 1
- Catalog number
- 011PUR001-00100 (100ug)
011PUR001-00500 (500ug)
011PUR001-01000 (1mg) - Description
-
Purotoxin1 (PT-1) is a peptide originally isolated from the Central Asian spider Geolycosa sp. It was shown to inhibit selectively P2X3 receptor channels at a 100 nM concentration. Studies were carried-out on cultured rat DRG neurons. Patch-clamp experiments did not show any inhibitory effect of PT-1 on voltage-gated channels (potentials range tested from -100 to 20 mV), neither on TRPV1 (after activation with 500 nM capsaicin). The selectivity of PT-1 for P2X3 was highlighted by activating this receptor with 10 uM ATP and 100 uM Alpha, Beta Methylene-ATP. Indeed, unlike P2X3, P2X2 and heterodimer P2X2/3 are known to be not sensitive to such concentrations. Moreover, P2X3, P2X2, and P2X2/3 are the only known ATP-sensitive receptors expressed in plasma membranes of DRG neurons. So, the observed effect seems to be well related to a selective inhibition of P2X3. P2X3-mediated current was fully inhibited with 100 nM PT-1, making it the most potent and selective ligand for P2X3.
- Source
- Smartox Biotechnology
- Product category
- Proteins & Peptides
- Product sub category
- Peptides
- Label
- No
- Shipment info
- Room Temperature
- Nacres Codification
- NA.26