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10 pull-downs EUR 160
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UbiTest Buffers Only

Product name
UbiTest Buffers Only
Catalog number
UM-0411-3000 (10 pull-downs)
Description
The UbiTest kit determines whether or not a target protein is poly-ubiquitinated. This powerful platform is an improvement on the most common method for investigating protein ubiquitination: immunoprecipitation and Western blot analysis. However, the substrate antibody often interacts differently with the poly-ubiquitinated forms of the substrate in the immuno-blotting step. This is due to epitope masking, reduced affinity, or changes in selectivity. A more definitive method for demonstrating protein ubiquitination is to couple immunoprecipitation with digestion by a broad spectrum deubiquitinase (DUB) prior to immunoblot analysis. An increased signal for the protein of interest (POI) after DUB treatment is a clear indication that the protein was ubiquitinated even if there was no clear reactivity in the untreated sample. To avoid potential problems arising from changes in immunoreactivity of the POI, the UbiTest assay utilizes TUBEs to pull-down the poly-ubiquitinated proteins. 
Source
LifeSensors
Product category
Lab Consumables
Product sub category
Buffers
Shipment info
Cool Pack
Nacres Codification
NA.25

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