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Product name: Animal-Free Recombinant Human TGF-β1 (CHO derived)
Catalog number: AF-100-21C-050 (50 ug)
Description: Recombinant Human TGF-ß1 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.
Purity: = 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity: Determined by TGF-ß1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The expected ED50 is = 0.05 ng/ml, corresponding to a specific activity of = 2 x 107 units/mg.
Source: Peprotech
Product category: Proteins & Peptides
Product sub category: Proteins
Shipment info: Room Temperature
Nacres Codification: NA.77

1. Authenticity: by N-terminal sequence analysis, and, when possible, by SDS-PAGE, RP-HPLC, and Mass Spectrometry analyses versus standards

2. Purity: by SDS-PAGE and RP-HPLC analysis

3. Biological Activity: by the relevant in vitro or in vivo bioassay

4. Protein content: by UV spectroscopy, SDS-PAGE analysis and, when possible, by HPLC analysis versus the calibrated standard solution

5. Endotoxin contamination: by kinetic LAL method

6. Microbiological contamination: by membrane filtration method; protein solutions are sterile-filtered prior to vialing

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