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500rxn EUR 744
744€

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Notch Reporter Assay Kit (cAMP/PKA Pathway)

Product name
Notch Reporter Assay Kit (cAMP/PKA Pathway)
Catalog number
60509 (500rxn)
Description
Notch Pathway Reporter kit is designed for monitoring the activity of Notch signaling pathway in the cultured cells. The kit contains the transfection-ready Notch1 NICD expression vector and CSL (CBF1/RBP-J) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized CSL responsive element upstream of a minimal promoter. The CSL (CBF1/RBP-J) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and back. 
Source
BPS Bioscience
Product category
Assays & Kits
Product sub category
Cell Based Assays
Application
Reporter Assay
Shipment info
Dry Ice
Nacres Codification
NA.84

Protocol

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