tebu-bio is a European company specialised in providing innovative reagents and laboratory services in Life Sciences
X

Need an answer now?

Give us a call

Leave a message, we'll get back to you





Move

Product added to your basket !


Continue your visit View your basket
110rxn EUR 574
574€

Outsource this assay to our lab

Ask here


Contact us

Recent History

SIRCOL Collagen Assay Kit

Product name
SIRCOL Collagen Assay Kit
Catalog number
S1000 (110rxn)
Description
Suitable for quantitatively monitoring collagen production during in-vitro cell culture or of recent in-vivo synthesis from tissue samples; such as wound healing and skin grafts research. Fibrillar collagens, [type I, II, III, V & XI] and basement membrane collagen, [type IV]. assay for acid-soluble, (cold 0.5 M acetic acid), mammalian collagens. 
Source
Biocolor
Product category
Assays & Kits
Product sub category
Simplex Quantification
Target species
Multi
Application
Colorimetry
Format
Ready-to-use
Shipment info
Room Temperature
Nacres Codification
NA.84

Download the datasheet

Download the PDF datasheet

Sircol FAQs

Sircol Frequently Asked Questions

Q. What is the sensitivity of the Sircol assay?

A. 1.0µg collagen.  Quantities as low as 1.0µg of collagen can be recovered from a 1 ml volume if the Collagen Isolation & Concentration protocol is used (See Sircol assay manual for more detail).

 

Q. How long does the Sircol assay take to run?

A. 1.5 hours

 

Q. What forms of collagen does the Sircol assay measure?

A. Acid-soluble and pepsin-soluble collagens.

 

Q. What types of collagen can the Sircol assay be used with?

A. Mammalian collagens, Types I to V.

 

Q. What absorbance values can samples be measured at?

A. Absorbance spectrum maximum for Sircol dye in alkali reagent is 555 nm however absorbance settings within the range 520-570 nm will give suitable readings.

 

Q. How do I prepare samples for measurement with the Sircol assay?

A. Preparation details for cell culture and tissue samples can be found in the assay manual.

 

The Sircol assay manual is the primary source of further information. It may also be helpful if the user familiarises themselves with some of the research papers in which the Sircol assay has been cited (see related documents in Sircol Technology Info).

Protocol

To access the protocol, please provide your email address :