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500µg EUR 172

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U-87 MG Whole Cell Lysate

Product name
U-87 MG Whole Cell Lysate
Catalog number
W09-001-GX2 (500µg)
. Application Note: Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µl depending on the size format of your gel.. Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8). . Concentration Value: 1.0 mg/ml. Concentration Definition: by modified Lowry assay. Purity and Specificity: HeLa cells were grown in Dulbecco?s medium supplemented with 10% fetal bovine serum. Cells were treated O/N with 0.2µg/mL of MG-132 in order to reduce degradation of ubiquitin-conjugated proteins by the proteasome. Cells were washed with PBS and then incubated on ic 
Product category
Cells, Media & Fractions
Product sub category
Cell Lysates & RNA
Western Blot,
Biological activity
Shipment info
Dry Ice
Nacres Codification

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