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500.0 µg EUR 158
158€


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A-172 Whole Cell Lysate

Product name
A-172 Whole Cell Lysate
Catalog number
W09-001-GL5 (500.0 µg)
Description
Background : Multi-purpose A-172 Whole Cell Lysates produced by Rockland Immunochemicals are derived from cell lines using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels.
Application Note : Multi-purpose A-172 Whole Cell Lysate are especially prepared as positive control for multiple assays including western blot, immunoprecipitation (IP), capture ELISA or other assays requiring native protein sample. For separation by SDS-PAGE and subsequent western blot analysis, lysates should be diluted by user to desired concentration in SDS-PAGE buffer with 2-mercaptoethanol or dithiothreitol as the reducing agent and heated to 95° C for 5 minutes. Sample is ready for use in immunoprecipitation and ELISA experiments, conditions should be optimized by the user. Rockland recommends its TrueBlot IP reagents for immunoprecipitation experiments. 
Source
Rockland
Product category
Cells, Media & Fractions
Product sub category
Cell Lysates & RNA
Application
ChIP, IP, WB
Label
(None)
Biological activity
 
Shipment info
Dry Ice
Nacres Codification
NA.61

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