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200.0 µg EUR 229
229€


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A431 Cell Nuclear Extract

Product name
A431 Cell Nuclear Extract
Catalog number
W09-001-A60 (200.0 µg)
Description
Background : Ready-to-use nuclear extracts produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
Application Note : Ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired add reducing agent prior to heating. The recommended loading volume per lane is 10-30 ?l depending on the size format of your gel. 
Source
Rockland
Product category
Cells, Media & Fractions
Product sub category
Cell Lysates & RNA
Application
WB
Label
(None)
Biological activity
 
Shipment info
Dry Ice
Nacres Codification
NA.61

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