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A549 Whole Cell Lysate

Product name
A549 Whole Cell Lysate
Catalog number
W09-001-372 (500ug)
Description
A549 Whole Cell Lysate SDS-Page tebu-bio Background : Ready-to-use whole cell lysates produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.  All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
Application Note : Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool.  If dissociating conditions are desired, add reducing agent prior to heating.  The recommended loading volume per lane is 10-20 µl depending on the size format of your gel. 
Source
Rockland
Product category
Cells, Media & Fractions
Product sub category
Cell Lysates & RNA
Target species
Human
Host
A549
Shipment info
Dry Ice
Nacres Codification
NA.61

Protocol

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A549 Whole Cell Lysate SDS-Page tebu-bioA549 Whole Cell Lysate SDS-Page tebu-bio