A431 Whole Cell Lysate

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Product name

A431 Whole Cell Lysate

Catalog number

W09-000-361 (500ug)

Description

Application Note : Ready-to-use A431 Whole Cell Lysate are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool.  If dissociating conditions are desired, add reducing agent prior to heating.  The recommended loading volume per lane is 10-20 µl depending on the size format of your gel. 

Source

Rockland

Product category

Cells, Media & Fractions

Product sub category

Cell Lysates & RNA

Target species

Human

Host

A431

Format

Cell Lysate

Shipment info

Dry Ice

Nacres Codification

NA.61