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A431 Whole Cell Lysate

Product name
A431 Whole Cell Lysate
Catalog number
W09-000-361 (500ug)
Description
Human Derived A431 Whole Cell Lysate - SDS-PAGE tebu-bio Application Note : Ready-to-use A431 Whole Cell Lysate are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool.  If dissociating conditions are desired, add reducing agent prior to heating.  The recommended loading volume per lane is 10-20 µl depending on the size format of your gel. 
Source
Rockland
Product category
Cells, Media & Fractions
Product sub category
Cell Lysates & RNA
Target species
Human
Host
A431
Format
Cell Lysate
Shipment info
Dry Ice
Nacres Codification
NA.61

Protocol

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Human Derived A431 Whole Cell Lysate - SDS-PAGE tebu-bio