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15ku EUR 221
221€


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Micrococcal Nuclease (S7)

Product name
Micrococcal Nuclease (S7)
Catalog number
MB-116-0015 (15ku)
Description
Background : Micrococcal nuclease catalyzes cleavage of both DNA and RNA to yield 3'-nucleotides. It exhibits exo- and endo-5'-phosphodiesterase activities. The enzyme catalyzes preferential endohydrolysis of the RNA and DNA at sites rich in adenylate or uridylate and deoxyadenylate or thymidylate. The enzyme has a molecular weight of 16,807 daltons and is calcium dependent. The pH optimum is 9.2 but varies depending upon the concentration of ionized calcium present.
Application Note : One Unit causes a change in optical density of 1.0 at 260 nm at 37°C, pH 8.0, using DNA as the substrate. ?6,000 units per mg protein. 
Source
Rockland
Product category
Genomics
Product sub category
Enzymes
Protein class
Nucleases
Shipment info
Cool Pack
Nacres Codification
NA.55

Protocol

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