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200ul EUR 309
309€


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Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP

Product name
Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP
Catalog number
18-8817-33 (200ul)
Description
Application Note : Mouse TrueBlot® ULTRA has been reported for use in IP/immunoblot and immunoblotting (WB). Mouse IgG TrueBlot® ULTRA is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. 
Source
Rockland
Product category
Antibodies
Product sub category
Secondary Antibodies
Target species
Mouse
Host
Rat
Clonality
Monoclonal
Application
WB, IP
Label
PO ULTRA
Shipment info
Cool Pack
Nacres Codification
NA.46

Protocol

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