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50ul EUR 150
150€


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Rabbit TrueBlot: Anti-Rabbit IgG HRP

Product name
Rabbit TrueBlot: Anti-Rabbit IgG HRP
Catalog number
18-8816-31 (50ul)
Description
Application Note : Rabbit IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Rabbit IgG TrueBlot® is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mLs/blot will yield enough reagent for 20 blots. Note that there are three key procedural considerations: 1. Protein A or G should not be used for the immunoprecipitation. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. For immunoprecipitation, Anti-Rat IgG beads, or Anti-Rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. 
Source
Rockland
Product category
Antibodies
Product sub category
Secondary Antibodies
Target species
Rabbit
Host
Mouse
Clonality
Monoclonal
Application
WB, IP
Label
PO ULTRA
Shipment info
Cool Pack
Nacres Codification
NA.46

Protocol

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