tebu-bio is a European company specialised in providing innovative reagents and laboratory services in Life Sciences
X

Need an answer now?

Give us a call

Leave a message, we'll get back to you





Move

Product added to your basket !


Continue your visit View your basket
50ul EUR 150
150€


Contact us

Sheep TrueBlot: Anti-Sheep IgG HRP

Product name
Sheep TrueBlot: Anti-Sheep IgG HRP
Catalog number
18-8815-31 (50ul)
Description
Application Note : Sheep IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Sheep IgG TrueBlot® is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 20 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the Mouse, Goat and Sheep TrueBlot secondaries, but not with the Rabbit TrueBlot secondary. Use of protein A or G beads with the Rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. 
Source
Rockland
Product category
Antibodies
Product sub category
Secondary Antibodies
Target species
Sheep
Host
Mouse
Clonality
Monoclonal
Application
WB
Label
PO ULTRA
Shipment info
Cool Pack
Nacres Codification
NA.46

Protocol

To access the protocol, please provide your email address :