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1kit EUR 435
435€

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ATPase ELIPA

Product name
ATPase ELIPA
Catalog number
BK051 (1kit)
Description
The assay is based upon an absorbance shift (330-360 nm) that occurs when 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is catalytically converted to 2-amino-6-mercapto-7-methyl purine in the presence of inorganic phosphate (Pi). The reaction is catalyzed by purine nucleoside phosphorylase (PNP). One molecule of inorganic phosphate will yield one molecule of 2-amino-6-mercapto-7-methyl purine in an essentially irreversible reaction. Thus, the absorbance at 360 nm is directly proportional to the amount of Pi generated in the reaction. - -The kit contains sufficient material for 96 assays. THe following components are included:- - 1. Reaction buffer- - 2. ELIPA reagent 1 (MESG)- - 3. ELIPA reagent 2 (PNP)- - 4. Phosphate standard- - 5. ELIPA reagent 1 resuspension buffer- - 6. ATP stock (Cat. nr BSA04)- - 7. GTP stock (Cat. nr BST06)- - 8. Manual with detailed protocols and extensive troubleshooting guide 
Source
Cytoskeleton
Product category
Assays & Kits
Product sub category
Functional Assays
Application
Colorimetry
Format
Ready-to-use
Shipment info
Room Temperature
Nacres Codification
NA.84

Download the datasheet

Download the PDF datasheet

Protocol

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