Actin Polymerization Kit

• Specifications
• Datasheet
• Tech Info

Product name

Actin Polymerization Kit

Catalog number

BK003 (1kit)

Description

The Actin Polymerization Biochem Kit is based on the enhanced fluorescence of pyrene conjugated actin that occurs during polymerization. The enhanced fluorescence that occurs when pyrene G-actin (monomer) forms pyrene F-actin can be measured in a fluorimeter to follow polymerization over time. Also, by using preformed pyrene F-actin, it is possible to follow depolymerization. Both cell/tissue extracts and purified proteins can be added to the reaction mixture to identify their effect on actin polymerization.- -The kit contains enough materials for 30-100 assays depending on assay volume. The following reagents are included:- - 1. 5 x 1 mg Pyrene labeled actin (Cat. nr AP05). - - 2. General Actin Buffer (Cat. nr BSA01).- - 3. Actin Polymerization Buffer (Cat. nr BSA02).- - 4. ATP 100mM (Cat. nr BSA04).- - 5. Tris-HCl pH 7.5, 100 mM- - 6. Manual with detailed protocols and extensive troubleshooting guide. 

Source

Cytoskeleton

Product category

Assays & Kits

Product sub category

Functional Assays

Application

Polymerisation Assays

Format

Ready-to-use

Shipment info

Room Temperature

Nacres Codification

NA.84

Download the datasheet

Download the PDF datasheet