Actin Polymerization Biochem Kit
- Product name
- Actin Polymerization Biochem Kit
- Catalog number
- BK003 (1kit)
- Description
-
The Actin Polymerization Biochem Kit is based on the enhanced fluorescence of pyrene conjugated actin that occurs during polymerization. The enhanced fluorescence that occurs when pyrene G-actin (monomer) forms pyrene F-actin can be measured in a fluorimeter to follow polymerization over time. Also, by using preformed pyrene F-actin, it is possible to follow depolymerization. Both cell/tissue extracts and purified proteins can be added to the reaction mixture to identify their effect on actin polymerization.- -The kit contains enough materials for 30-100 assays depending on assay volume. The following reagents are included:- - 1. 5 x 1 mg Pyrene labeled actin (Cat. nr AP05). - - 2. General Actin Buffer (Cat. nr BSA01).- - 3. Actin Polymerization Buffer (Cat. nr BSA02).- - 4. ATP 100mM (Cat. nr BSA04).- - 5. Tris-HCl pH 7.5, 100 mM- - 6. Manual with detailed protocols and extensive troubleshooting guide.
- Source
- Cytoskeleton
- Product category
- Assays & Kits
- Product sub category
- Functional Assays
- Application
- Polymerisation Assays
- Format
- Ready-to-use
- Shipment info
- Room Temperature
- Nacres Codification
- NA.84