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1kit EUR 1071
1071€

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Actin Polymerization Kit

Product name
Actin Polymerization Kit
Catalog number
BK003 (1kit)
Description
The Actin Polymerization Biochem Kit is based on the enhanced fluorescence of pyrene conjugated actin that occurs during polymerization. The enhanced fluorescence that occurs when pyrene G-actin (monomer) forms pyrene F-actin can be measured in a fluorimeter to follow polymerization over time. Also, by using preformed pyrene F-actin, it is possible to follow depolymerization. Both cell/tissue extracts and purified proteins can be added to the reaction mixture to identify their effect on actin polymerization.- -The kit contains enough materials for 30-100 assays depending on assay volume. The following reagents are included:- - 1. 5 x 1 mg Pyrene labeled actin (Cat. nr AP05). - - 2. General Actin Buffer (Cat. nr BSA01).- - 3. Actin Polymerization Buffer (Cat. nr BSA02).- - 4. ATP 100mM (Cat. nr BSA04).- - 5. Tris-HCl pH 7.5, 100 mM- - 6. Manual with detailed protocols and extensive troubleshooting guide. 
Source
Cytoskeleton
Product category
Assays & Kits
Product sub category
Functional Assays
Application
Polymerisation Assays
Format
Ready-to-use
Shipment info
Room Temperature
Nacres Codification
NA.84

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Protocol

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