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Product name
Catalog number
CL3005 (5x5ml)
CL3010 (100ml)
Standard Rabbit Complement is derived from a pool of normal rabbit serum. It is intended for use as a source of complement for any of the standard lymphocytotoxicity procedures utilizing human peripheral blood lymphocytes (PBL's), including HLA-ABC tissue typing. The Rabbit Complement is added to human PBL's that have been pre-incubated with anti-HLA antisera. The sensitized cells are incubated in the presence of Rabbit Complement for the appropriate length of time, and then scored for degree of killing. Full details of all the standard lymphocytotoxicity techniques are published in the NIAID Manual of Tissue Typing Techniques. 
Cedarlane Laboratories Limited
Product category
Cells, Media & Fractions
Product sub category
Target species
Shipment info
Dry Ice
Nacres Codification

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Complement FAQs

1.  You have several different complement formulations available. How do I know which type to use?

This decision is based on what cells you need to lyse and from what species the cells are from. Typically any type of complement should lyse cells, however we have made different formulations to enhance performance and reduce non-specific toxicity in certain assays. Listed below (and in our catalogue) are some general guidelines:

    Our Standard Rabbit Complement is mainly used for HLA-ABC tissue typing or cytotoxicity assays using human T cells.
    Our Low-Tox® complement formulations are processed to reduce the toxicity of the complement. They are used for cells that may be sensitive to non-specific lysis. Low-Tox-H® is mainly for HLA-DR tissue typing (human B cells), Low-Tox-M® is for cytotoxicity assays using mouse lymphocytes, and Low-Tox-R® is for cytotoxicity assays using rat lymphocytes.
    Cedarlane’s Baby Rabbit Complement and Baby Rabbit Complement-Tissue Culture Grade are used for cytotoxicity assays which requires the C’ to have low toxicity while maintaining a relatively high activity (higher than the Low-Tox® formats, but lower that the standard rabbit C’). Furthermore, the Baby Rabbit Complement-Tissue Culture Grade can be used for assays requiring the C’ to be sterile, virus and mycoplasma-free, and low in endotoxin.
    Our Rabbit Complement MA is screened for use in cytotoxicity assays using monoclonal antibodies against human lymphocytes.
    Cedarlane’s Standard Guinea Pig Complement is mainly used for hemolytic plaque assays and complement fixation assays. The Hemo-Lo format is used for sheep RBC hemolytic assays, and the Low-Tox® GPC’ is mainly for cytotoxicity and plaque assays.

2.  What is special about the Low-Tox® formulations?

This type of complement will have low background toxicity (i.e. few cells will be lysed non-specifically).

3.  What dilutions should I normally use?

This varies in each assay, therefore we recommend that you titrate to find your optimal dilution. Generally, a 1:8-1:16 dilution should be suitable (1:2-1:4 should be used for microlymphocytoxicity/tissue typing assays). Additional information may be found on the product data sheet.

4.  What is the shelf life of your complement?

Generally, the expiry is 2 years from date of manufacture for all of our complement formulations.

5.  Can I use expired complement for my assay?

You may be able to use complement that has recently expired, however we cannot guarantee its performance. Should you decide to try it, we recommend using more (ie. lower dilution) due to possible loss of activity.

6.  Can I re-use my reconstituted complement?

Yes. If the complement is frozen to -70°C (in single use aliquots) immediately after reconstitution it can be used, however only one freeze / thaw cycle is recommended.

7.  The isotype of my primary antibody will not fix complement. Can I use a secondary antibody that fixes complement to deplete cells labeled with this primary antibody?

This has been tried in the past but with limited success, therefore we do not recommend it.

8.  How do I sterilize complement for assays requiring sterile product?

You can sterile filter your complement using a 0.22 um filter, HOWEVER you must dilute your complement first to your optimal dilution before filtering. Otherwise, you may lose complement activity because of the filtration process. You can also use the Baby Rabbit Complement-Tissue Culture Grade which is sterile.

9.  Is it better to use frozen or lyophilized complement?

There is no difference in quality. Lyophilized complement should be stored at –20C, and frozen complement must be stored at –80C. The lyophilized product will last longer, is more stable, and is easier to ship (dry ice is required to ship frozen complement).


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