Evaluate in vitro metabolism of drug candidates
Subcellular fractions are widely used in drug discovery and preclinical drug development to evaluate the in vitro metabolism of drug candidates. Metabolic stability, in vitro intrinsic clearance, reaction phenotyping and CYP/UGT enzyme inhibition can all be evaluated with subcellular fractions.
The primary subcellular fractions used to support these studies are microsomes, S9, cytosol and mitochondria. XenoTech offers these fractions from humans and toxicologically relevant species.
Standard Subcellular Fraction Products:
Standard Organs Available
custom subcellular fractions can be easily ordered to meet the specific needs of any study.
Contact your dedicated product specialist to find out how our custom products can meet your needs.
Advantages of Using XenoTech’s Subcellular Fractions
- Large donor pools minimize lot-to-lot variation and increase the long-term availability of each lot
- All lots are prepared and characterized according to Standard Operating Procedures
- The quality of all subcellular fractions is governed by the assigned Study Director, the Study Manager and XenoTech’s Quality Control Unit
- Preparation procedures allow for the production of matching S9 and microsomal donor pools
- Characterization assays for human pools are performed under conditions to obtain Vmax value
- CYP and UGT activity characterization assays are performed with XenoTech’s automated liquid handling and incubation system along with LC/MS/MS analysis
- Enzymatic activity assays are end-point assays performed in triplicate with saturating concentrations of the probe substrate
- Established acceptance criteria and positive controls determine the acceptability of lots