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CleanAmp Primers

CleanAmp Primers contain thermolabile chemical modifications that allow for primer-based Hot Start activation in PCR. These modifications prevent primer extension at the lower temperatures of PCR set-up and manipulation. A Hot Start thermal activation step removes the modification and generates the corresponding unmodified primer, which supports amplification of the desired target.

Developing an Assay?

Introducing CleanAmp Primers as your Hot Start solution can be 0.05 EUR per assay or less. Trilink guarantee at least 10 ODs per primer synthesis, which is enough material for approximately 6,250 reactions. At 400 EUR per primer pair, your added cost per reaction is just 0.05 EUR to replace your standard primers with CleanAmp Primers.

Which CleanAmp Primer is right for my application?

CleanAmp Primers are available in two forms, which vary in the rate of thermal activation. CleanAmp Turbo modifications are released more quickly than those for CleanAmp Precision. The two different speeds of primer activation are suited for a number of PCR-based applications.  

CleanAmp Turbo Primers CleanAmp Precision Primers
  • Fast cycling
  • Standard cycling
  • Multiplex PCR
  • One-step reverse-transcription PCR
  • Improves amplicon yield
  • Improved specificity and limit of detection
  • Reduces mis-priming/primer dimer formation
  • Greatest reduction in mis-priming/primer dimer formation

More information

Please use the Related Downlaods section on the right of this page to download

  • Application note
  • Product manual
  • FAQs

 You can also visit the page related to CleanAmp dNTPs here.

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