tebu-bio is a European company specialised in providing innovative reagents and laboratory services in Life Sciences
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Recombinant protein production and purification platform

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Our expertise ranges from molecular biology, multiplex studies to large scale production of purified recombinant proteins. We are specialized in recombinant protein production, antibodies included, for downstream R&D applications (e.g. functional assay, selection of variants, crystallography...).

Your recombinant protein production project

  • Outsource a part of or your entire protein production project
  • Ideal tool to speed up your protein-based research program
  • Initial feasibility study to ensure selection of the most appropriate strategy
  • Fully customizable service platform for R&D-scale protein purification from prokaryotic (E. coli) or eukaryotic (Hek293 EBNA) cells

Example of a standard project

  • Production optimization step: expression study (multiplex format)
  • Protein production from small to large scale
  • Purification optimization steps: screening of different chromatography strategies and binding-elution conditions (multiplex purification)
  • Protein purification by affinity, ion exchange and/or gel filtration chromatography on AKTA FPLC system
  • QCs and delivery: Coomassie gel, Western Blot, quantitation, aliquoting of sample, Endotoxin level testing (FDA approved LAL test)

Project Management

  • Process expertise
    Steps are customizable and allow for complete project outsourcing in an ISO9001-2008 certified quality management environment
  • Complete transparency
    Project updates are provided weekly by a designated Project Manager and a specific Sales Manager is available for business interactions
  • No strings attached
    Process methods are made available to customers for fast and efficient industrial exploitation with preservation of IP rights

 

Recently, tebu-bio produced in vitro truncated tau fragments containing the four microtubule binding repeat domain with a P301L mutation in E. coli. This custom recombinant protein has been used in a recent publication by Janssen R&D for the development of a complete tauopathy model associated with neurodegeneration and inflammation. Peeraer et al. Neurobiol Dis. 2015 Jan; 73: 83–95. DOI: 10.1016/j.nbd.2014.08.032

 

Image credits: PDB ID 3D90 (monomer and dimer), 5AUU, 4TPR