Cloning & sub-cloning

Cloning

Save time and effort by outsourcing your Molecular Biology project to tebu-bio. Together, we will define the best customised strategy to meet your requirements.

•  Modularity
  Each project is divided into modules and go/no go steps for more flexibility & security

•  Customisation
  Fully customised projects to match your requirements

•  Follow-up
  A dedicated project manager for regular updates on your project

• Confidentiality
  Strict confidentiality rules

•  Quality Controls & Standard Operating Procedures (SOPs)      

We can help you with your cloning strategy, whether we start from your ready-to-use insert or whether we help you to customise your vector construction for protein expression…

•  Insert: cDNA, PCR product, oligos/adapters, microDNA or RNA

•  Vector: any plasmid supplied by the researcher

Basic cloning steps:

• DNA restriction & purification

• Ligation & E.coli transformation

• Screening (minipreps or PCR)

• Restriction profile & maxiprep

Customised vector construction:

•  Basic cloning steps

• Insert engineering by PCR amplification of supplied cDNAs (tags, endopeptidases, signal peptide…), multiple cloning steps.

• Quality control: double-strand sequencing of full insert