Adipose tissue is comprised of precursor cells called preadipocytes and adipocytes (fat cells). Stimulation of the preadipocytes, in the subcutaneous skin layer, to differentiate into fat cells is called adipogenesis. By studying products capable of triggering or enhancing the differentiation of preadipocytes into fat cells, one can produce a cosmetic product designed to work with a person’s own body to naturally fill in deficient areas of the skin. For example, the creation of fat cells in an aged face can provide a natural augmenter to fill out the fine lines and wrinkles of the face. Skin would have increased thickness and volume thereby resulting in smoother, younger looking skin.
This quantitative bioassay allows the measurement of triglyceride concentration following a 7-day compound treatment of human preadipocytes. The end point assay is total triglyceride content of the cells 2 weeks after initiation of human preadipocyte differentiation. The triglyceride concentration is determined from the concentration of glycerol released after cell lysis and triglyceride hydrolysis.
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