Anti-Oxidant screening services

Why anti-oxidant screens?

We offer two screening methods for evaluating the total antioxidant activity of compounds or extracts: ABTS and ORAC antioxidant assays. Both assays can determine the total antioxidant capacity of biological fluids, cells, tissue, and naturally occurring or synthetic compounds for use as dietary supplements, topical protection, and therapeutics.

Our offer

ABTS Assay

The ABTS assay measures ABTS.+ radical cation formation induced by metmyoglobin and hydrogen peroxide. Trolox [6-Hydroxy-2,5,7, 8-tetramethylchroman-2-carboxylic acid], a water soluble vitamin E analog, serves as a positive control inhibiting the formation of the radical cation in a dose dependent manner. The antioxidant activity in biological fluids, cells, tissues, and natural extracts can be normalized to equivalent Trolox units to quantify the composite antioxidant activity present. This assay measures radical scavenging by electron donation.

ORAC Assay

The ORAC (Oxygen Radical Absorbance Capacity) antioxidant assay measures the loss of fluorescein fluorescence over time due to peroxyl-radical formation by the breakdown of AAPH (2,2.-azobis-2-methyl-propanimidamide, dihydrochloride). Trolox [6-Hydroxy-2,5,7, 8-tetramethylchroman-2-carboxylic acid], a water soluble vitamin E analog, serves as a positive control inhibiting fluorescein decay in a dose dependent manner. The ORAC assay is a kinetic assay measuring fluroescein decay and antioxidant protection over time. The antioxidant activity in biological fluids, cells, tissues, and natural extracts can be normalized to equivalent Trolox units to quantify the composite antioxidant activity present. This assay measures antioxidant activity by hydrogen atom transfer.

It's easy!

Just send us your compound in DMSO (no structure information or compound name requested, only a small quantity is required). You'll receive a full study report, including raw data.