CleanTag™ Library preparation for Next-Generation Sequencing
What are the benefits of the CleanTag™ technology?
Small RNA sequencing (small RNA-seq) is a powerful tool, however library preparation is dominated by adapter dimer formation. Adapter dimers form when the 5΄ and 3΄ adapters self-ligate without the target library insert. Consequently, amplification of this smaller adapter dimer side product often out-competes amplification of the tagged library.
A unique solution (TriLink Biotechnologies) employing CleanTag™ modified adapters, blocks adapter-adapter ligation thus improving the adapter dimer to tagged library ratio, which leads to significantly higher quality sequencing data.
This patent-pending technology:
- Increases valuable sequencing reads
- Handles RNA inputs as low as 1 ng
- Tags efficiently even in samples with low miRNA abundance
- Eliminates the need for gel purification
- Makes it possible to achieve full automation
CleanTag™ renders small RNA-Seq fully automatable
The size of the adapter dimer and tagged library are often similar in length, thus requiring a gel size selection to enrich the libraries for the desired product. Additionally, most commercially available library prep kits require large inputs or a gel enrichment step prior to ligation. Because of this, a fully automated workflow was not possible. TriLink's CleanTag™ technology eliminates the need for enrichment and gel purification, thus allowing full automation of small RNA-seq for the first time.