Lentifect™ Purified Lentivirus
Lentiviral vectors provide potent vehicles for delivery of genes into a wide range of cell types including difficult-to-transfect cells. Lentiviral particles transduce cells and integrate into the host genome in dividing and post-mitotic cells resulting in long-term expression of the transgene both in vivo and in vitro. Producing, concentrating and titrating lentiviral particles, however, is time consuming and requires some experience to achieve high titers and consistent results. tebu-bio has selected GeneCopoeia, for their scientific expertise in reliably and efficiently producing viral particles.
High titer lentivirus are produced from standardized protocols using high-purity plasmid DNA preparations and EndoFectin™ Lenti transfection and TiterBoost™ reagents. High quality reagents and GeneCopoeia's scientific expertise ensure production of the highest-titers possible of Standard viral supernatant and purified particles.
Read the Technical Note by Ed Davis "Lentivirus: that's my MOI, and I'm sticking to it"
Download the Technote here
Transduction of H1299 cells with GeneCopoeia eGFP lentiviral particles
H1299 cells (ATCC) in 24-well plates were transduced with the indicated amounts of eGFP purified particles, catalog number LPP-EGFP-LV105 in the presence of 5 µg/ml of polybrene. The expression of eGFP was checked with a fluorescence microscope 72 hours post-transduction.
Ready-for-transduction, Lentifect™ offers the following advantages:
A cost-effective choice for busy labs
A reliable lentiviral source for consistent lot-to-lot quality
Efficient transduction of difficult-to-transfect cells or animal models
Offer valid up to March 31st, for more information contact your local tebu-bio office. Non-cumulative with any other discounts, special offers or price agreements.