Cell Biology and Signalling
Focus on Cytoskeleton

MARCH 2016

NEWS HIGHLIGHT

Live Cell Imaging with SiR-actin, SiR-tubulin & SiR-DNA

Launched just over a year ago, SiR-Actin and SiR-Tubulin have been available to European researchers providing the most convenient tools to stain F-actin and Microtubules in living cells.

In the meantime, Spirochrome have launched a third stain based on the SiR-technology: SiR-DNA to stain DNA in living cells.

Which results have other researchers obtained with these stains?

PRODUCT FOCUS

Tubulin polymerisation measurement – made easy!

In this post, our specialist focuses on methods for measuring the dynamic polymerisation of tubulin to microtubules and to detect the impact of compounds or other variables in your experiments on this process.

Cytoskeleton Inc. has developed and commercialized a set of tubulin polymerisation assays which are adapted to different experimental set-ups and questions. 

Find the assay best suited to your model

Tubulin: Microtubule ratio measured in cells

How can you measure changes of the ratio between free tubulin and filamentous microtubules in cells? The method described here is based on the most reproducible and accurate method of determining the amount of microtubule content versus free-tubulin content in a cell population.

Discover the Microtubules/Tubulin in ViVo Assay Kit

Versatile measurement of protein binding to microtubules

Do you suspect your protein might bind to microtubules? That it might stabilize or destabilize these filamentous structures?

If so, then this post will help you to find a meaningful assay to validate your assumption.

Measure microtuble binding capabilities of proteins of interest

PROMOTIONS

Choose 2 tubulin items and get 15% discount

  • Polymerization Assays
  • Binding Assays
  • Pre-formed Microtubules
  • Pure, active Proteins

Valid up to April 30th 2016 - Please mention CA0361-037 on your order to benefit from this discount.

Choose 2 or more items from this list and get 15% discount

TECHNICAL CORNER

Oxidative stress is based on the imbalance between the production of free radicals and the ability of the body to detoxify their hazardous effects through neutralization by antioxidants. Free radicals and reactive oxygen species (ROS) are highly reactive molecules that are generated by normal cellular processes, environmental stress, and UV irradiation. Organisms have developed complex antioxidant systems to protect themselves from oxidative stress, however, excess ROS can overwhelm the systems and cause severe damage.

2 versatile methods to measure the anti-oxidative potential of your sample

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