08/12/11 - PDE1B Cell-Based Reporter Assay Kit

Background

PDE1B plays a key role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE1B hydrolyzes both cAMP and cGMP to nucleoside 5`-monophosphate and prefers cGMP at low substrate levels.

Description

The PDE1B Cell-Based Reporter Assay Kit (cat# 60601) is designed for screening inhibitors of PDE1B in cultured cells. The kit is also ideal for monitoring PDE1B/ cGMP/ Ca2+ channel signaling pathway activity in vivo. It is useful in determining transfection efficiency. The kit includes a recombinant HEK293 cell line that stably expresses human PDE1B, atrial natriuretic peptide (ANP), mammalian expression constructs for ANP receptor A, CNGA2, and aequorin, and buffer for the assay.

References

1. Yu, J. et al. (1997). “Identification and Characterization of a Human Calmodulin-Stimulated Phosphodiesterase PDE1B1“ Cell Signaling 9(7): 519-529.
2. Bender, A.T. et al., (2005). “Selective up-regulation of PDE1B2 upon monocyte-tomacrophage differentiation.” PNAS 102(2): 497-502.
3. Wunder, F. et al., (2009). “A novel PDE2A reporter cell line: characterization of the cellular activity of PDE inhibitors.” Mol. Pharm. 6(1): 326-36.

Figure 1. Schematic presentation of cGMP assay used to characterize the function of PDE1B.