13/12/11 - RARalpha Reporter (Luc) - HEK293 Cell Line

Background

RAR belongs to a family of nuclear receptors and has three subtypes, RARa, RARb, and RARg.
RAR heterodimerizes with RXR (retinoid X receptor) and acts as a transcription factor that regulates the growth and differentiation of both normal and malignant cells. When RAR binds to its ligands, all-trans retinoic acid or 9-cis retinoic acid, RAR/ RXR heterodimer binds to retinoic acid response elements in the promoter region of target genes and recruits coactivator proteins, leading to transcription and expression of the downstream target genes.

Description

The RARalpha Reporter (Luc)-HEK293 Cell Line (cat# 60503) is designed for monitoring the activity of retinoic acid receptor alpha (RARa). The RARalpha Reporter (Luc)-HEK293 Cell Line contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARa (GenBank Accession No. NM_000964). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid. The expression of RARa is confirmed by Western blotting.

Applications

• Monitor RARa-regulated pathway activity
• Screen agonists or antagonists of RARa.

Format

Each vial contains ~2 million cells in 1 ml of 10% DMSO.

References

1. Petkovich, M, et al. Nature (1987) 330(6147): 444-450.
2. Allenby, G, et al. Proc. Natl. Acad. Sci. USA (1993) 90(1): 30-34.

Figure 1. Dose response of RARalpha Reporter (Luc) - HEK293 cells to all-trans retinoic acid (ATRA).