19/05/11 - Platforms for Neurobiology

Neuronal Cell Lines

Hypothalamic Cell Lines

CELLutions Biosystems offers a unique line of phenotypically different clones generated from:
Adult Mouse Hypothalamic cells: 23 cell lines designated mHypoA-xx (Cat. # CLU172 - CLU194)
Embryonic Mouse Hypothalamic cells: 38 cell lines designated mHypoE-xx (Cat. # CLU101 - CLU139)
Embryonic Rat Hypothalamic cells: 22 cell lines designated rHypoE-xx (Cat. # CLU201 - CLU222)

Based on a proprietary platform technology, these hypothalamic neuronal cell lines have been created by immortalizing hypothalamic primary cultures using retroviral transfer of SV40 T-Ag. These cell lines have been found to express an ever expanding array of neuropeptides, enzymatic markers and biologically active receptors including: Neuropeptide Y, Oxytocin, POMC, Ghrelin, Metastatin/Kisspeptin, Arg Vasopressin, Leptin Receptor (ObRb), GHS-R, Estrogen alpha and beta Receptors, Serotonin Receptor and Neurotensin.

Hippocampal Cell Lines

This line of embryonic mouse hippocampal cell lines was derived in a similar fashion as described for the embryonic hypothalamic lines resulting in four phenotypcially distinct cell lines designated mHippoE-xx (CLU196-CLU199)

Pituitary Cell Lines

22 phenotypically different cell lines designated mPitA-xx (Cat. # CLU401-CLU423)

This line of adult mouse pituitary cell lines is based on a proprietary platform technology which has enabled the creation of 19 mixed cell cultures that contain cells from particular pituitary cell lineages, as determined by RT-PCR analysis and immunocytochemistry for specific hormones. Pituitary cell cultures have been immortalized from fully differentiated adult mouse pituitary cell culture (C57Bl/6; female) by retroviral transfer of SV40 T-Ag.

mPitA-14 cell line                                    mPitA-15 cell line
- Prolactin (PRL)                                     - Oxytocin (OXT)

The hypothalamic, hippocampal and pituitary cell lines are easy to culture, have efficient transfection rates (the embryonic cells more so than the adult cells) and have robust gene and protein expression. As such, these cell lines enable accurate in vitro assays for use in the discovery, development and validation of new therapeutics targeted to central-nervous system diseases and disorders, including: obesity, stress, reproduction and metabolic disorders, amongst others.