Genome-wide cellular model for in vitro gene silencing

The availability of cellular models with silenced genes offers the possibility of hands-on studies based on RNA interference (RNAi). Access to such cell lines helps the researcher, to focus on experimental questions without the need to first construct a cellular model. tebu-bio offers SilenciX adherent cells that have been modified by non-viral and safe transfection for long-term silencing of genes by RNAi.

The SilenciX technology is based on three unique properties:

• A non-integrative plasmid leading the production of a few episomal copies per cell
• A powerful algorithm to design target specific shRNAs coupled to a genome-wide blast screening making the SilenciX technology robust
• A virus-free in vitro liposomal transfection system

SilenciX cell lines benefits

Ready-to-use silenced cell lines: Save time on cell preparation, transfection and selection

High specificity: High knock-down with no off-target effects

Cost-saving: No need to buy expensive transfection reagents

Robust cell model with no off-target effects: No need to validate the silencing effect of your repeated siRNA experiments

Guaranteed knock-down: More than 70% extinction at RNA level (data shown on data sheet)

Stable: Extinction seen over more than 500 days for highly reproducible results

Proven: Tried, accepted and used as an in vitro model in many research labs (see publications list in related downloads section)

Each kit contains:

• 1 vial of "target specific" SilenciX cells, validated by qPCR
• 1 vial of control SilenciX cells (transfected with a non-relevant shRNA sequence)
• a complete user manual and specification sheets for each cell line

 Catalogued items or custom options

SilenciX cell lines already available from catalogue (HeLa cell line)

If you'd like to silence a specific gene in HeLa cells not yet available on our list, following a feasibility study we'll be able to start production for the same advantageous price as our catalogued items.

Possible customisation for both cell types and targets (Human or Murine)

For other Human or Murine cell lines, our product specialists will be more than pleased to suggest solutions for your project (request on line).

SilenciX is a registered trademark of tebu-bio, Technology licensed from CEA.