CleanAmp dNTPs contain thermolabile modifications that present an alternate Hot Start activation approach for PCR. This latest development shows great promise as a general Hot Start solution, allowing for easy implementation into your existing protocols.

A New Approach to High Stringency PCR

CleanAmpdNTPs offer precise control at the start of PCR thermal cycling by blocking DNA polymerase nucleotide incorporation until heat activated. The temperature dependent control of DNA polymerase extension vastly reduces mis-priming, primer dimer formation and other common deleterious effects (see Figure 1 of  the gallery on the right side). This improvement in the specificity of target amplification allows for enhanced detection of low input template concentrations (Figure 2 in the gallery).

A Flexible Solution

CleanAmp dNTPs are compatible with a number of thermostable DNA polymerases, allowing for great flexibility in PCR design (Figure 3 in the gallery). The use of CleanAmp dNTPs in the experiment shown in Figure 3 eliminated or reduced primer dimer formations. Moreover, all of the DNA polymerases were able to produce the desired amplicon demonstrating the versatility of CleanAmp dNTPs.

Improved PCR Performance

When CleanAmp dNTPs are used in combination with other Hot Start solutions, a greater benefit can be achieved (Figure 5 in the gallery). CleanAmp dNTPs can also be used in conjunction with our other CleanAmp Product, the Primers, to offer even greater control of your reaction. An improvement in PCR performance can be seen, especially with CleanAmp Precision Primers, where off-target amplification was entirely eliminated. Although each Hot Start approach improves the specificity and efficiency of PCR reactions, this improvement can be further enhanced when used in combination with CleanAmp dNTPs.