Target-specific silenced cell lines available for:

Drug discovery	Functional studies	HTS
Target identification	Pathway detection	Assay development
Target validation	Drug screening	ADME-Tox

Ready-to-use SilenciX HeLa cell lines are available as catalog items (click here for the full list)

Wish to discuss SilenciX or reserve your cell lines today? 
Our product specialist Aurélie SERRA, PhD will be pleased to help you.

NEW ! SilenciX is now available to researchers in the USA !
To find out more, please contact Shankar Sellappan, PhD at SABiosciences (Frederick, MD).

Argaunote 2	DICER 1	ICAM-1 receptor	SIRT 1	TRAILR 2
Bcl-2	DROSHA	MAPK 1	STAT 1	WNK 1
BAX	Exportin 5	MTOR	Sorcin
c-myc	FADD	Notch 2	TGFB1
Caspase 3	GPS 1	Ntsr 2	TNFR1
COX 2	HDAC 1	PKC	TRAF 6

 

Human genome-wide SilenciX for target studies

The availability of cellular models with silenced genes offers the possibility of hands-on studies based on RNA interference (RNAi). Access to such cell lines helps you, the researcher, to focus on experimental questions without the need to first construct a cellular model. tebu-bio offers SilenciX human adherent cells that have been modified by non-viral and safe transfection for long-term silencing of genes by RNAi.

tebu-bio has developped catalogued silenced HeLa cell lines. We are continuously expanding our SilenciX list to meet your research needs and invite any suggestions of new human target knock-down to put at the top of our SilenciX production. Feel free to contact your local office or our product specialist  Aurélie SERRA, PhD to discuss your requirements.

Custom construction services using our SilenciX technology are also available in the human cell line of your choice with your gene of interest.

Each kit contains:

• 1 vial of "target specific" SilenciX cells, validated by qPCR
• 1 vial of control SilenciX cells (transfected with a non-relevant shRNA sequence)
• a complete user manual and specification sheets for each cell line

SilenciX cell lines benefits

Robust cell model with no off-target effects

• New algorithm called DSIR (Designer of Small Interfering RNA) for predicting siRNA efficacy based on a simple linear model
• Best performing method among existing algorithms (Matveeva et al, 2007)
• High specificity of shRNA sequence to the target

Guaranteed knock-down

• shRNA-based technology
• More than 70% extinction at RNA level (data shown on data sheet)

No vector integration in cell genome

• Virus-free system
• Low copy number per cell

Stable silencing 

• Long-term efficiency (>500 days) => see recurrent publications with the same cell line

SilenciX publications 

Expression of the trkA or TrkB receptor tyrosine kinase alters the double-strand break (DSB) repair capacity of SY5Y neuroblatsoma cells

Schulte et al., DNA repair Amsterdam 2008

PARP inhibition vs. PARP-1 silencing different outcomes in terms of single-strand break repair and radiation susceptibility
Godon et al., Nucleic Acids Res. 2008; 36(13):4454-64

Moderate variations in CDC25B protein levels modulate the response to DNA damaging agents
Aressy et al., Cell Cycle. 2008;Jul;7(14):2234-40

Role of ATM in the telomere response to the G-quadruplex ligand 360A
Pennarun et al., Nucleic Acids Res. 2008; 36(5):1741-54

Interplay between cernunnos-XLF and NHEJ proteins at DNA ends in the cell
Wu et al., J. Biol. Chem. 2007;282(44):31937-43

Untangling the relationships between DNA repair pathways by silencing more than 20 DNA repair genes in humn stable clones.
Biard, Nucleic Acids Res. 2007 June; 35 (11): 3535–3550

Long-term XPC silencing reduces DNA double-strand break repair
Despras et al., Cancer Res 2007 March; 67 (6): 2526–2534

For more information please contact our product specialist Aurélie SERRA, PhD.

SilenciX is a trademark of tebu-bio.

Technology licensed from 

The DSIR algorithm was developped jointly by the Center for Computational Biology at the Ecole des Mines de Paris and the Laboratoire BIM (Biologie, Informatique et Mathematique) at the CEA.