ELISA AND IFA KITS

The immunofluorescence antibody assay (IFA) are used as a method of detecting antibodies to a particular viral antigen.
Our kits are reliable for not only detecting the presence of antibodies specific for HHV-6, -7 and -8 , but also for visualizing the cellular localization of the antigen of interest.

Our enzyme-linked immunosorbent assay (ELISA) specific for HHV-6, -7 and -8 makes it much easier to screen large numbers of samples.


FRET FLUORIMETRIC PROTEASE ASSAYS & RELATED REAGENTS

Viral proteases are identified as an important drug-screening target. HIV and HCV Proteases are key targets for antiviral drugs. Identification of protease inhibitors and development of new drugs interacting with viral proteases correspond to huge financial stakes. The use of robust and sensitive assays to screen HIV and HCV proteases is then crucial for companies involved there. The EnzoLyte™ Protease Assay Kits use an optimized FRET peptide substrate for the continuous measurement of HCV or HIV protease activities.


OUR SELECTION OF ASSAYS
1/ HCV Protease Assay Kits for HTS of HCV NS3/4A protease inhibitors and for continuous detection of this protease.

2/ HIV Protease Assay Kits for HTS of HIV protease inhibitors and for continuous detection of this protease that cleaves the precursor polyprotein.

The assays are available with various FRET peptides; wavelength used for the detection being different.
HIV assays: 490nm (cat. nr 71127) or 520 nm (cat. nr 71147) .

HCV assays: 490nm (cat. nr 71126), 520 nm (cat. nr 71145) and 620 nm (cat. nr 71146) .



RELATED PRODUCTS
1/HIV and HCV related peptides:
These peptides can be proteases substrates, inhibitors or specific for main viral glycoproteins (HIV).

To know more about our viral peptide offer, please click here.

2/E. Coli recombinant protease:
HCV NS3/4A protease
HIV Protease

NEW !

FRET WEST NILE VIRUS PROTEASE ASSAY

SensoLyte™ West Nile Virus Protease Assay Kits provide a convenient, homogeneous assay for high throughput screening of West Nile Virus protease NS3 inhibitors.
Utilizing a AMC-labeled substrate (SensoLyte™ 440 West Nile Virus Protease Assay Kit) or a FRET peptide (SensoLyte™ 570 West Nile Virus Protease Assay Kit), these assays provide continuous quantification of protease activity. Upon NS3 protease cleavage, the fluorescence of AMC in the former and 5-TAMRA in the latter is recovered, and monitored at their characteristic emission wavelength. In the SensoLyte™ 570 West Nile Virus Protease Assay Kit, a novel FRET substrate containing 5-TAMRA as a fluorophore and QXL™ 570 as a quencher was developed by AnaSpec. The long wavelength fluorescence of 5-TAMRA shows less interference from autofluorescence of cell components and test compounds, thus making it more sensitive than the AMC-labeled substrate.

SensoLyte^TM 440 West Nile Virus Protease Assay Kit
SensoLyte^TM 570 West Nile Virus Protease Assay Kit

Related Product:
West Nile Virus NS3 Protease, recombinant