Virology & Microbiology
Take advantage of these high quality DNA / RNA controls for Nucleic Acid Tests (NAT) (1-3). DNA / DNA controls (together with Viral lysates) are ideal companion for PCR reactions (including Nested-PCR). The quantitated controls have been characterized in such a way that the number of DNA copy per µl is known. They are suitable fpr quantitative PCR. To a lower extend the non quantitated controls might also be used for such a purpose by assessing the number of copies / ml according to its MW (see table below).
A few examples of the latest publications describing the use of some of them: 1 Jebbink et al. Development of Real-Time PCR Assays for the quantitative detection of Epstein-Barr Virus and Cytomegalovirus, Comparison of TaqMan Probes, and Molecular Beacons. JMD 2003; Vol 5, 1.
2 Stöcher et al. Parallel detection of five human herpes virus DNAs by a set 3: 85-93.
3 Sun et al. Detection and genotyping of human herpes simplex viruses in cutaneous lesions of Erythema Multiforme by nested PCR. J Med. Virol. 2003; 71 : 423-428.
Genome Size and Copies/ml of DNA/RNA Controls
| Organism
|
Genome MW | Copies/ml @ 50 ng/ml | cat number |
|
CMV |
1.65 x 108 |
1.8 x 108 |
|
|
EBV |
1.15 x 108 |
2.6 x 108 |
|
|
HHV-6 |
1.08 x 108 |
2.8 x 108 |
|
|
HSV-1 |
1.01 x 108 |
3.0 x 108 |
|
| HSV-2 |
1.01 x 108 |
3.0 x 108 |
|
| Chlamydia trachomatis |
6.94 x 108 |
4.3 x 107 |
|
|
Toxoplasma gondii |
5.94 x 1010 |
5.1 x 105 |
|
A few examples of the latest publications describing the use of some of them: 1 Jebbink et al. Development of Real-Time PCR Assays for the quantitative detection of Epstein-Barr Virus and Cytomegalovirus, Comparison of TaqMan Probes, and Molecular Beacons. JMD 2003; Vol 5, 1.
2 Stöcher et al. Parallel detection of five human herpes virus DNAs by a set 3: 85-93.
3 Sun et al. Detection and genotyping of human herpes simplex viruses in cutaneous lesions of Erythema Multiforme by nested PCR. J Med. Virol. 2003; 71 : 423-428.
Latest Products
| Name | Size | Ref. | Price (excl. VAT) |
| ADENOVIRUS-5 DNA CONTROL | 100 ul | 08-728-000 | 640.00 € |
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| Name | Size | Ref. | Price (excl. VAT) |
| BK VIRUS DNA CONTROL | 250 ul | 08-942-250 | 690.00 € |
| CMV AD169 DNA CONTROL | 250 ul | 08-925-000 | 640.00 € |
| HSV-1 MACINTYRE DNA CONTROL | 250 ul | 08-921-000 | 640.00 € |
| JC VIRUS DNA CONTROL | 250 ul | 08-943-250 | 690.00 € |
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